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Isolation and characterisation of a yeast chromosomal replicator

Nature volume 282, pages 39–43 (1979)Cite this article

Abstract

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.

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References

  1. Maaløe, O. & Kjeldgaard, N. O. Control of Macromolecular Synthesis (W. A. Benjamin, New York, 1966).

    Google Scholar 

  2. Hemstetter, C., Cooper, S., Diorucci, O. & Revelas, F. Cold Spring Harb. Symp. quant. Biol. 33, 809–822 (1968).

    Article  Google Scholar 

  3. Carins, J. J. molec. Biol. 6, 208–213 (1963).

    Article  Google Scholar 

  4. Jacob, E., Brenner, B. & Cuzin, F. Cold Spring Harb. Symp. quant. Biol. 28, 329–347 (1963).

    Article  CAS  Google Scholar 

  5. Cold Spring Harb. Symp. quant. Biol. 43 (1978).

  6. Hand, R. Cell 15, 317–325 (1978).

    Article  CAS  Google Scholar 

  7. Sheinin, R. & Humbert, J. A. Rev. Biochem. 47, 277–316 (1978).

    Article  CAS  Google Scholar 

  8. Pardee, A. B., Dubrow, R., Harmlin, J. L. & Kletzien, R. F. A. Rev. Biochem. 47, 715–750 (1978).

    Article  CAS  Google Scholar 

  9. Struhl, K., Stinchcomb, D. T., Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 1035–1039 (1979).

    Article  ADS  CAS  Google Scholar 

  10. McDonnell, M. W., Simon, M. N. & Studier, F. W. J. molec. Biol. 110, 119–146 (1977).

    Article  Google Scholar 

  11. Hinnen, A., Hicks, J. B. & Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).

    Article  ADS  CAS  Google Scholar 

  12. Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. (in the press).

  13. Hicks, J. B., Hinnen, A. & Fink, G. R. Cold Spring Harb. Symp. quant. Biol. 43, 1305–1313 (1978).

    Article  Google Scholar 

  14. Wensink, P. C., Finnegan, D. J., Donelson, J. E. & Hogness, D. S. Cell 3, 315–325 (1974).

    Article  CAS  Google Scholar 

  15. Hiraga, S. Proc. natn. Acad. Sci. U.S.A. 73, 198–202 (1976).

    Article  ADS  CAS  Google Scholar 

  16. Yasuda, S. & Hirota, Y. Proc. natn. Acad. Sci. U.S.A. 74, 5458–5462 (1977).

    Article  ADS  CAS  Google Scholar 

  17. Struhl, K. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 74, 5255–5259 (1977).

    Article  ADS  CAS  Google Scholar 

  18. Struhl, K., Fink, G. R. & Davis, R. W. Nature 279, 78–79 (1979).

    Article  ADS  CAS  Google Scholar 

  19. Struhl, K. & Davis, R. W. J. molec. Biol. (in the press).

  20. Newlon, C. S., Petes, T. D., Hereford, L. M. & Fangman, W. K. Nature 247, 32–35 (1974).

    Article  ADS  CAS  Google Scholar 

  21. Petes, T. D., Newlon, C. S., Byers, B. & Fangman, W. L. Cold Spring Harb. Symp. quant. Biol. 38, 9–16 (1973).

    Article  Google Scholar 

  22. Petes, T. D. & Williamson, D. H. Expl Cell Res. 95, 103–110 (1975).

    Article  CAS  Google Scholar 

  23. Burke, W. & Fangman, W. L. Cell 5, 263–269 (1975).

    Article  CAS  Google Scholar 

  24. Hartwell, L. H. Bact. Rev. 38, 164–198 (1974).

    CAS  PubMed  Google Scholar 

  25. Southern, E. J. molec. Biol. 98, 503–577 (1975).

    Article  CAS  Google Scholar 

  26. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

  27. Cameron, J. R., Loh, E. Y. & Davis, R. W. Cell 16, 739–751 (1979).

    Article  CAS  Google Scholar 

  28. Struhl, K., Cameron, J. R. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 1471–1475 (1976).

    Article  ADS  CAS  Google Scholar 

  29. Bolivar, R., et al. Gene 2, 95–113 (1977).

    Article  CAS  Google Scholar 

  30. McClintock, B. Proc. natn. Acad. Sci. U.S.A. 18, 677–681 (1932), 25, 405–416 (1939).

    Article  ADS  CAS  Google Scholar 

  31. Lis, J. T., Prestidge, L. & Hogness, D. S. Cell 14, 901–917 (1978).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Stanford University School of Medicine, Stanford, California, 94305

    D. T. Stinchcomb, K. Struhl & R. W. Davis

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  1. D. T. Stinchcomb
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  2. K. Struhl
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  3. R. W. Davis
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Stinchcomb, D., Struhl, K. & Davis, R. Isolation and characterisation of a yeast chromosomal replicator. Nature 282, 39–43 (1979). https://doi.org/10.1038/282039a0

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